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What is the principle of interpretation of the UV-Vis spectrometer?

Author:QINSUN Released in:2023-05 Click:66

The ultraviolet-visible spectrometer irradiates the analyzed organic substance with ultraviolet-visible light of a certain frequency, causing the transition of valence electrons in the molecule, which will be selectively absorbed.

Set of spectra whose absorption varies with wavelength, reflecting the characteristics of the sample.

The wavelength range involved in the ultraviolet-visible spectrometer is 0.2 to 0.8 microns, and it is widely used in organic chemistry research.

Usually used for substance identification, purity testing, and organic molecular structure research.

In terms of quantification, it can determine the content of various components in compounds with complex structures and mixtures, and can also determine the dissociation constant of substances, stability constant complexes, the moleculeweight identification and indicates end point in microtiter and as a detector in liquid chromatography etc.

The ultraviolet-visible spectrometer can measure many substances:

Trace antimony in pure tin-free copper, mercury in tea, bismuth in pure aluminum metallic materials, bismuth in pure nickel metallic materials, bismuth in raw lead, palladium, platinum, iridium in catalyst, zinc in rice flour, arsenic in baking soda, zinc in rice, manganese and molybdenum in low alloy steel, copper in groundwater, cadmium, geological cobalt in samples, chromium and total chromium in electroplating wastewater, cadmium, bismuth in electrolytic copper, l silver in fixer, cerium group rare earths in monazite, mercury in sea hydride catalyst leach solution wastecure, cerium group rare earths in wastewater, gold, cadmium, total amount of cerium group rare earths in copper and steel, vanadium, cobalt, aluminum, molybdenum, niobium, titanium, tin, yttrium, total amount of rare earths, tantalum in superalloys, vanadium, cadmium, mercury, cobalt, nickel, grains in industrial wastewater Thorium in waste solution, tin in canned foods, barium in silicon-barium inoculant, thorium in silicon-magnesium alloy, palladium in secondary noble metal alloy, bismuth in copper-containing sample, cobalt in catalyst synthesis of ammonia, molybdenum in a steel alloy, etc.

When selecting various instruments, there are certain standards, such as measurement accuracy or measurement range. When selecting a UV-Vis spectrometer, we take into account the confioptical guration, spectral range, sample type and analytical tools.

Optical structure mainly refers to whether the light emitted from the UV spectrophotometer is a single beam or a double beam. The single beam is measured by a single beam of light, the wavelength is given when measuring, then the light absorption result is obtained through the measured object and the control object.

The double beam splits the beam in two using a chopper wheel. Light sources include infrared, ultraviolet, and visible light.

Tungsten and halogen lamps generally cover only the part of the visible light. Xenon lamps can cover both ultraviolet and visible light regions.

The ultraviolet oil measuring instrument is developed according to the national ultraviolet spectrophotometric oil measuring method HJ970-2018environmental water quality monitoring system, combined with the environmental pollution situation in my country and the environmental monitoring needs of departments at all levels;

It is an instrument relatively efficient, environmentally friendly, convenient and fast oil measuring device. H970 UV oil meter has stable performance and powerful functions, which can meet users\' application needs.