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Your location: Home > Related Articles > interprets the problems and solutions that are likely to occur when using chromatography columns-

interprets the problems and solutions that are likely to occur when using chromatography columns-

Author:QINSUN Released in:2023-05 Click:110

The common problem in the use of chromatographic columns is that the column pressure increases. If the column pressure increases slowly during long-term use, it is a normal phenomenon. However, the column pressure suddenly increases during use (except for the blockage of the system pipeline and the failure of the pressure sensor, the possible reasons are as follows:

1. The pressure plate filter screen at the top of the chromatographic column is clogged or contaminated;
2 1. The load at the top of the chromatographic column is contaminated with the sample;
3. The salt of the buffer solution in the column chromatography encounters a high concentration of methanol or other organic solvents, forming crystallization;
4. The pH value of the mobile phase is too high or too low to destroy or dissolve the stationary phase structure.

The solution is the followinge:
1 If the filter screen plate at the top of the chromatographic column is determined to be contaminated, the column can be flushed with methanol in the reverse direction of normal pressure, or remove the chromatographic column head , put it in 10% dilute nitric acid and ultrasonically clean it for 10 minutes, then use pure water to sonicate for 10 minutes, then reinstall the chromatography column.
2. If there is is determined that the chromatographic column head packing is contaminated, remove the screw from the column head, dig up the contaminated packing in the front section of the column, pack it with the same column packing, and reinstall the screw to column head after careful repair. -methanol concentration.
4. If the pH value is misused, it is difficult to retrieve.

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