Types of small molecule compounds commonly used in organoid culture

Organoid is a kind of 3D in vitro cell culture system, a model that closely resembles the source tissue or organ in vivo. These 3D in vitro culture systems can replicate the complex spatial morphology of differentiated tissues and can study the relationship between show cells among themselves and between cells and their surrounding matrix Interaction and spatial position morphology And it can have similar physiological responses to differentiated tissues and organs in vivo, and has high similarity to source tissues Compared with traditional 2D cell culture models, 3D cultured organoids contain a variety of cell types, breaks the simple physical contact between cells, grows stem cells in matrigel, and cultivates corresponding organs under the influence of chemical small molecule inhibitors/activators, cytokines, medium additives and other substances.can be better used to simulate the developmental process and the physiological and pathological conditions of organ tissues. Therefore, it basically has broad application in research and clinical diagnosis and treatment.

Small molecules refer to compounds with a molecular weight less than 1000 daltons (especially less than 400 daltons) and biological functions. Such compounds differ from cytokines and proteins, which can pass directly through the cell membrane and enter cells, and then perform similar functions, and are suitable for cells of different lineages. Small molecular compounds have many purposes and are widely used in many important areas in life sciences, such as stem cells, organoids, immunology, neurobiology, epigenetics, apoptosis, ion channels, oncology and signal transduction.

Small molecule compounds commonly used in organoid culture are:

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