1. Is it necessary to wash the cells with PBS?
We know that in the \"adherent cell passage\" the serum in the medium inhibits the activity of trypsin and affects Digestive effect, so should be washed with PBS. However, no information has been found for cell replacement that clearly indicates whether PBS washing is required. I think it should be considered according to the specific situation of the cell:
1. If the cells are relatively \"delicate\" or if growth is not particularly good, it is recommended not to wash with PBS.
<br style=\"box-sizing: border-box; color: rgb(51, 51, 51); font-family:\"Helvetica Neue\", Helvetica, Tahoma, Arial, sans-serif; font size: 16px; white space: normal; backgroundcolor: rgb(255, 255, 255); The state may not be very firm and washing with PBS will destroy the adherent state of the cells; on the other hand, PBS may wash away some of the growth factors secreted by the cells, which is also not conducive to the adjustment of the cells\' state. can be washed slowly to reduce damage to cells
2. When the cells grow well, or the cell solution is not very clean under the microscope, you can choose to wash with PBS.
On the one hand, it can remove the accumulated metabolites; Some cells with poor growth status or ageing are washed out to preserve cell vitality.
2. What is \"Cell Half Exchange\"<br style=\"box-sizing: border-box; color: rgb(51, 51 , 51); font-family: \" Helvetica Neue\", Helvetica, Tahoma, Arial, sans serif; font size: 16px; whitespace: normal; background-color: rgb(255, 255, 255);\"/
\"Cell half replacement\" is also called \"cell half replaceing\", meaning that half of the old medium is discarded and half of the new medium is added. The reason for choosing it is actually a bit like the reason for not adding PBS.
The reasons are:
1. Provide cells with an adaptive buffer environment;
2. Some growth factors can be secreted during cell growth to promote growth.
3. Save material
4. Convenient operation: Like a 96-well plate, it is very inconvenient to change the liquid, so often use a driving gun to change half of the liquid
5. For suspension cell cultures, half-change medium is sometimes used to reduce cell density
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