Notes on the use of liquid chromatography columns

A liquid chromatographic column is an instrument used for liquid chromatographic analysis.

(1) Precautions for using the liquid chromatography column:

There is no special instruction for the storage liquid of the liquid chromatography column and it is the mobile phase that is shown in the evaluation report. Before use, make sure that the liquid chromatography column storage solution is miscible with the mobile phase of the sample to be analyzed. In reversed-phase chromatography, if high concentration salt or buffer is used as the eluent, an eluent with a low concentration of about 10% in the organic phase must be used for the transition, otherwise the salt in the buffer will settle in the organic phase. high concentration phase. phase It is easy to separate and block the chromatographic column.

(2) Mobile phase:

The various organic solvents that are present in the mobile phase are used, should be as chromatographically pure as possible Glassware of doubly distilled water. It would be better if the prepared mobile phase is filtered through a 0.45 μm filter membrane, especially the mobile phase containing salt. In addition, devices such as mobile phase containers and in-line filters in the chromatographic system must be regularly cleaned or replaced.

The usual pH range for bonded phase packing based on conventional silica gel is 2.0-8.0. When the chromatographic column is to be used under the boundary conditions of the applicable pH value range, it must be cleaned immediately after each use with a solvent suitable for storage of chromatographic columns and compatible with the mobile used, and the original replace spent mobile phase.

(3) Monster:

The monster should also be as clean as possible. You can use a sample filter or a sampleuse pre-treatment column (spe) to pre-treat the sample; if the sample is difficult use a protective column to handle. When analyzing samples with normal phase chromatography, all solvents and samples must be strictly dehydrated.

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