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Your location: Home > Related Articles > Moisture Resistance Microbial Penetration Tester Experiment

Moisture Resistance Microbial Penetration Tester Experiment

Author:QINSUN Released in:2023-08 Click:39

The Moisture Resistance Microbial Penetration Tester is used to measure the performance of materials in resisting the penetration of bacteria into liquids when subjected to mechanical friction (the performance of protection against moisture). penetration of bacteria carried by liquids when subjected to mechanical friction), and is mainly used in medical operations Sheets, surgical gowns and clean clothes, etc., Qisnun Precision Electromechanical Technology Co., Ltd. is the manufacturer and welcomes customers who need to inquire.

Applicable standards:

YY/T 0506.2, YY/T 0506.6-2009, EN ISO 22610

Main parameters:

1 , Turntable speed: (60±1) rpm;

2. Test pressure: 3N±0.02N;

3. Wheel speed toward outside: 5~6rpm;

4. Timer setting range: 0~99.99min;

5. Total weightinner and outer ring weights: 800 g ± 1 g.

Experiment Description:

There are 5 main stages in the testing experiment:

The First Step: Preparation of Bacteria Sheet

Staphylococcus aureus ATCC29213 was cultured on tryptic soy agar at 36°C ± 1°C for 18-24h, inoculated 2-3 colonies in 3ml of tryptic soy broth and inoculated at 36°C Cultivate at ℃±1℃ for 18-24h. Dilute with peptone water 1:10 to a concentration of 1 × 104 CFU/mL ~ 4 × 104 CFU/mL and count the bacterial suspension.

Open the aseptic bag and remove the polyurethane film that is still attached to the IQ, and place the wettable polyurethane film from the bacteria-carrying material sheet on the clean plate. For ease of use, use double-sided tape. Attach the four corners of the bacteria-carrying material sheet to the plate. Use thee Petri dish lid as a template to cut out a corresponding area on the bacterial film, spread 1.0ml of Staphylococcus aureus suspension on this area, and then dry the bacteria sheet at 56°C for about 30 minutes, using sterilized glass during drying. the applicator continues to spread the bacteria suspension over the Bacteria-Carrier Film to evenly distribute the bacterial fluid. Bacterial flakes were prepared and used the same day.

Step 2: Condition adjustment

If necessary, adjust the sample condition according to GB6529, or perform the condition limit and test under temperature conditions standard normal ambient. The condition adjustment method shall be recorded in the test report.

Step 3: Adjusting the test

Adjust the weight on the control rod so that the force applied by the test finger on the agar is 3N±0.02N. Place an agar dish on the trayturning.


Use the following counts: Take a circular weight consisting of inner and outer rings, with a total weight of 800g ± 1g exerts a standard tension on the material. Place the cylinder in the center of the inner ring, then cover the cylinder and the inner ring with the test tube, and place the bacterial flake with the stained side down on the test tube after removing the test tube. sticky. A layer of HDPE film is covered over the polyurethane film and the outer ring is firmly pushed down so that the three layers of material are firmly added between the two rings.

Step 5: Perform the test

Carefully place the above ring on a Petri dish of agar with the lid removed, the steel ring hangs freely from the outside. outside of the rotating disk, place the test finger on the HDPE membrane inside the mouth of the can so that thespecimen is in contact with the surface of the agar. The test consists of applying a pressure of 3 N in accordance with the above regulations and the instrument operates for 15 minutes. Immediately after 15 minutes, the ring set was removed and set aside. Remove a Petri dish from the centrifuge and replace the lid. Immediately place the second Petri dish and its ring on the rotating disk.

Perform the above steps with the same set of rings for the following four petri dishes.

After the five petri dishes were tested, the bacteria slices were removed and discarded, and the specimens were turned over and covered with HDPE film inside out.

Operate on the sixth petri dish for 15 minutes to complete a parallel test group.

The remaining 4 samples were analyzed in the same manner as above in six petri dishes for 15 minutes each, and each sample was tested.Use a piece of freshly prepared bacterial slice.

If there is liquid on the surface of the agar, place it on a clean bench to dry, cover each plate with agar and place it at 36°C±1°C for 48 hours.

Count the number of Staphylococcus aureus colonies in each petri dish, and the number of colonies within 15 minutes of the center of the petri dish is not counted.