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Your location: Home > Related Articles > Dry engineering goods: Will sealant quality be damaged after freezing?

Dry engineering goods: Will sealant quality be damaged after freezing?

Author:QINSUN Released in:2023-04 Click:19

We know that a complete ELISA kit contains:

①The enzyme\'s substrate;

②The carrier in the solid phase (immunoadsorbent) coated with antigen or antibody;

③Enzyme labeling Antigen or antibody (conjugate) ;

④Conjugate and sample dilution;

⑤ Wash solution, in plate ELISA, the usual diluent used is phosphate buffered saline with 0.05% Tween 20;

⑥ Negative control and positive control product (in qualitative assay), reference standard and control serum (in quantitative determination);

⑦Enzyme reaction termination solution, commonly used HRP reaction termination solution is sulfuric acid, and the concentration varies according to the added amount and volume of thee colorimetric Generally, 2mol/L is used in plate ELISA.

Will the quality of the frozen kit be compromised? Hengyuan Biology will answer for you:

It can be saved Use , but do not freeze and thaw repeatedly. If you are doing experiments within a week, you can store them at 2-8°C; if you are doing experiments within a week to a month, you can store them at -20°C; if you do experiments during one month, Can be stored at -80°C. But it is worth noting that the greater loss of freezing and thawing than 2-8 ℃ storage loss is mainly due to protein degradation, and the protein will be broken down once it freezes and thaws.

In setting up the ELISA method for reaction kinetic studiesek, It shows that the two antigen-antibody reactions are generally for 1 to 2 hours at 37 ° C, and the product can naturally reach the ding peak. The temperatures commonly used for incubation are 43°C, 37°C, room temperature and 4°C, etc. When incubating at room temperature, it should be placed flat on the console. 37°C is the commonly used heat preservation temperature in the laboratory, and it is suitable temperature for most antigen-antibody binding. The method of heat preservation is generally a water bath, except that some ELISA instruments are equipped with a special electric heating block. The ELISA plate can be placed in a water bath box. The bottom of the ELISA plate should be attached to the water surface to quickly equilibrate the temperature. The room temperature during operation should be strictly limited within the specified range. The default room temperaturer refers to 20-25 °C, but incubation can be controlled according to manual requirements during specific operations.

Hengyuan Biology has a group of professional and experienced technical engineers, after years of accumulation, the kits now cover many types and more than a thousand indicators, with the characteristics of fast, simple and accurate, meeting the needs of universities and scientific research personnel, and improving the work efficiency of scientific research personnel. mature and stable quality has won the recognition of many scientific research institutions, and the perfect stock and delivery system ensures that all products can be supplied from stock. I look forward to your consultation.